NGS: a revolution in DNA sequencing!
Next-generation sequencing (NGS) is revolutionizing the field of molecular biology. At Conidia Coniphy, we put our technical and scientific expertise at your service to offer customized NGS sequencing solutions tailored to your specific needs.
Next-generation sequencing: the definition!
Next-generation sequencing (NGS) is revolutionizing DNA analysis. By producing massive data from millions of DNA fragments simultaneously, NGS is making sequencing faster and more cost-effective.
This technology, mainly based on illumina synthesis sequencing, produces short DNA reads. Thanks to its strength, NGS has become an indispensable tool in many fields of biology, from fundamental research to personalized medicine.
NGS sequencing: how does it work
NGS technology involves several stages, from sample preparation to results analysis. Here are the main phases of a case study at CONIDIA CONIPHY: Monitoring fungicide resistance of plant diseases.
Sample preparation
The DNA to be analyzed is extracted and purified.
Amplification
Each fragment (gene) is copied several times to guarantee better reading.
Sequencing
Fragments are sequenced simultaneously, producing a large amount of data.
Data analysis
The results are then compiled and processed using powerful algorithms, enabling the identification of mutations, genetic variations or other key information.
Proprietary Analysis Pipeline
CONIDIA CONIHY develop project specific and easy-to-use bioinformatic tools.
Why the choice of Conidia Coniphy for NGS analysis?
Innovation at the heart of our organization.
Our R&D team of doctors and engineers is dedicated to innovative projects and the development of state-of-the-art equipment and practical tools (molecular biology kits) to meet the current challenges in the field.
Our DNA includes:
- Recognized expertise in molecular biology and NGS techniques.
- Customized solutions tailored to your specific projects.
- Full support from sample preparation to results analysis.
- We are at your service to guide you and help you make the most of the various sequencing techniques for your research.
NGS: the evolution of Sanger sequencing
The study of DNA has been revolutionized by advances in sequencing. Sanger sequencing, a pioneer in the field, paved the way for the human genome project.
This project stimulated research, leading to the emergence of high-speed sequencing (NGS). More efficient, NGS generates massive quantities of data, requiring sophisticated bioinformatics tools for analysis.
Compared to Sanger, NGS offers significantly improved sequencing capacity, enabling the simultaneous analysis of a large number of DNA molecules.
The NGS method enriched by long-read sequencing (ONT)
ONT (Oxford Nanopore Technologies) long-read sequencing is a 3rd generation NGS sequencing technology, offering significant advantages for specific applications. By combining the strengths of these two technologies (illumina and ONT), researchers can obtain a more complete and accurate view of genomes.
This method is based on:
Analysis of complex regions
of the genome that cannot be well resolved by short-fragment sequencing methods.
Study structural variations,
repeats and rearrangements in the genome with greater precision.
Offer a more complete and detailed view
of the genome, useful in applications such as whole-genome sequencing, the study of epigenetic modifications, and metagenomics.
The method of choice
for projects requiring in-depth, comprehensive genome analysis.
Contact us for a customized sequencing
If you’re looking to better understand or exploit NGS technology, Conidia Coniphy is your trusted partner. We offer solutions tailored to your next-generation sequencing needs.
Please contact us to discuss your projects and find out how we can support your research.
Our solutions associated with NGS technology
idetect
Phytosanitary product testing
Let's work together !
If you have any needs or questions about environmental microbiology, contact us and we'll provide you with the answers you need.